Install ampliconnoise by entering the following commands in the terminal:
sudo apt update sudo apt install ampliconnoise
removal of noise from 454 sequenced PCR amplicons
AmpliconNoise is a package of applications to clean up high-throughput sequence data. It consists of three main parts: . Pyronoise - does flowgram-based clustering to spot misreads SeqNoise - removes PCR point mutations Perseus - removes PCR chimeras without the need for a set of reference sequences . Previously there was a standalone "Pyronoise" by the same authors and this package includes an updated version. There is also a "Denoiser" in Qiime which is related but distinct.